<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Filichkin, S. A.</style></author><author><style face="normal" font="default" size="100%">Megraw, M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing</style></title><secondary-title><style face="normal" font="default" size="100%">Methods Mol Biol</style></secondary-title><short-title><style face="normal" font="default" size="100%">Methods in molecular biology (Clifton, N.J.)</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2017</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://link.springer.com/protocol/10.1007/978-1-4939-7125-1_10</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">1629</style></volume><pages><style face="normal" font="default" size="100%">141-154</style></pages><isbn><style face="normal" font="default" size="100%">1940-6029 (Electronic)1064-3745 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Identifying cis-regulatory elements is critical in understanding the direct and indirect interactions that occur within gene regulatory networks. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high-throughput sequencing to identify regions of regulatory DNA on a genome-wide scale. Yet, challenges still remain in processing recalcitrant tissues that have low DNA content. Here, we describe DNase I SIM (for Simplified In-nucleus Method), a protocol that simplifies and facilitates generation of DNase-seq libraries from plant tissues for high-resolution mapping of DNase I hypersensitive sites. By removing steps requiring the use of gel agarose plugs in DNase-seq, DNase I SIM reduces the time required to perform the protocol by at least 2 days, while also making possible the processing of difficult plant tissues including plant roots.&lt;/p&gt;</style></abstract></record></records></xml>