01445nas a2200145   4500008004100000020004600041245009100087210006900178300001200247490000900259520092600268100002201194700001501216856006801231       2017                        eng d  a1940-6029 (Electronic)1064-3745 (Linking)00aDNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing0 aDNase I SIM A Simplified InNucleus Method for DNase I Hypersensi  a141-1540 v16293 a<p>Identifying cis-regulatory elements is critical in understanding the direct and indirect interactions that occur within gene regulatory networks. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high-throughput sequencing to identify regions of regulatory DNA on a genome-wide scale. Yet, challenges still remain in processing recalcitrant tissues that have low DNA content. Here, we describe DNase I SIM (for Simplified In-nucleus Method), a protocol that simplifies and facilitates generation of DNase-seq libraries from plant tissues for high-resolution mapping of DNase I hypersensitive sites. By removing steps requiring the use of gel agarose plugs in DNase-seq, DNase I SIM reduces the time required to perform the protocol by at least 2 days, while also making possible the processing of difficult plant tissues including plant roots.</p>1 aFilichkin, S., A.1 aMegraw, M.  uhttps://link.springer.com/protocol/10.1007/978-1-4939-7125-1_10