01715nas a2200253   4500008004100000022001400041245011200055210006900167260001300236300001100249490000700260520091500267653002701182653002501209653002101234653003401255653001501289653002601304100002201330700002101352700001801373700002101391856004901412       2015                        eng d  a1879-035600aAlternative splicing in plants: directing traffic at the crossroads of adaptation and environmental stress.0 aAlternative splicing in plants directing traffic at the crossroa  c2015 Apr  a125-350 v243 a<p>In recent years, high-throughput sequencing-based analysis of plant transcriptomes has suggested that up to &sim;60% of plant gene loci encode alternatively spliced mature transcripts. These studies have also revealed that alternative splicing in plants can be regulated by cell type, developmental stage, the environment, and the circadian clock. Alternative splicing is coupled to RNA surveillance and processing mechanisms, including nonsense mediated decay. Recently, non-protein-coding transcripts have also been shown to undergo alternative splicing. These discoveries collectively describe a robust system of post-transcriptional regulatory feedback loops which influence RNA abundance. In this review, we summarize recent studies describing the specific roles alternative splicing and RNA surveillance play in plant adaptation to environmental stresses and the regulation of the circadian clock.</p>10aAdaptation, Biological10aAlternative Splicing10aCircadian Clocks10aPlant Physiological Phenomena10aRNA, Plant10aStress, Physiological1 aFilichkin, Sergei1 aPriest, Henry, D1 aMegraw, Molly1 aMockler, Todd, C  uhttps://megraw.cgrb.oregonstate.edu/node/31402854nas a2200373   4500008004100000022001400041245012300055210006900178260001600247300001100263490000800274520174600282653001602028653002502044653001802069653002302087653002802110653001902138653001602157653001702173100002502190700001802215700001802233700001902251700001602270700002102286700002102307700001802328700002202346700002502368700001802393700002002411856004902431       2015                        eng d  a1477-912900aThe cyclophilin A DIAGEOTROPICA gene affects auxin transport in both root and shoot to control lateral root formation.0 acyclophilin A DIAGEOTROPICA gene affects auxin transport in both  c2015 Feb 15  a712-210 v1423 a<p>Cyclophilin A is a conserved peptidyl-prolyl cis-trans isomerase (PPIase) best known as the cellular receptor of the immunosuppressant cyclosporine A. Despite significant effort, evidence of developmental functions of cyclophilin A in non-plant systems has remained obscure. Mutations in a tomato (Solanum lycopersicum) cyclophilin A ortholog, DIAGEOTROPICA (DGT), have been shown to abolish the organogenesis of lateral roots; however, a mechanistic explanation of the phenotype is lacking. Here, we show that the dgt mutant lacks auxin maxima relevant to priming and specification of lateral root founder cells. DGT is expressed in shoot and root, and localizes to both the nucleus and cytoplasm during lateral root organogenesis. Mutation of ENTIRE/IAA9, a member of the auxin-responsive Aux/IAA protein family of transcriptional repressors, partially restores the inability of dgt to initiate lateral root primordia but not the primordia outgrowth. By comparison, grafting of a wild-type scion restores the process of lateral root formation, consistent with participation of a mobile signal. Antibodies do not detect movement of the DGT protein into the dgt rootstock; however, experiments with radiolabeled auxin and an auxin-specific microelectrode demonstrate abnormal auxin fluxes. Functional studies of DGT in heterologous yeast and tobacco-leaf auxin-transport systems demonstrate that DGT negatively regulates PIN-FORMED (PIN) auxin efflux transporters by affecting their plasma membrane localization. Studies in tomato support complex effects of the dgt mutation on PIN expression level, expression domain and plasma membrane localization. Our data demonstrate that DGT regulates auxin transport in lateral root formation.</p>10aArabidopsis10aBiological Transport10aCyclophilin A10aIndoleacetic Acids10aLycopersicon esculentum10aPlant Proteins10aPlant Roots10aPlant Shoots1 aIvanchenko, Maria, G1 aZhu, Jinsheng1 aWang, Bangjun1 aMedvecká, Eva1 aDu, Yunlong1 aAzzarello, Elisa1 aMancuso, Stefano1 aMegraw, Molly1 aFilichkin, Sergei1 aDubrovsky, Joseph, G1 aFriml, Jiří1 aGeisler, Markus  uhttps://megraw.cgrb.oregonstate.edu/node/316