01333nas a2200145   4500008004100000245008400041210006900125260001200194300001200206490000900218520084900227100002501076700001801101856006801119       2018                        eng d00aNanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing0 aNanoCAGEXL An Approach to HighConfidence Transcription Start Sit  c07/2018  a225-2370 v18303 a<p>Identifying the transcription start sites (TSS) of genes is essential for characterizing promoter regions. Several protocols have been developed to capture the 5&prime; end of transcripts via Cap-Analysis of Gene Expression (CAGE) or linker-ligation strategies such as Paired-End Analysis of Transcription Start Sites (PEAT), but often require large amounts of tissue. More recently, nanoCAGE was developed for sequencing on the Illumina GAIIx to overcome this limitation. In this chapter, we present the nanoCAGE-XL protocol, the first publicly available adaptation of nanoCAGE for sequencing on recent ultra-high-throughput platforms such as Illumina HiSeq-2000. NanoCAGE-XL provides a method for precise transcription start site identification in large eukaryotic genomes, even in cases where input total RNA quantity is very limited.</p>1 aIvanchenko, Maria, G1 aMegraw, Molly  uhttps://link.springer.com/protocol/10.1007/978-1-4939-8657-6_13