@inbook {743,
	title = {DNase I SIM: A Simplified In-Nucleus Method for DNase I Hypersensitive Site Sequencing},
	booktitle = {Methods Mol Biol},
	volume = {1629},
	year = {2017},
	pages = {141-154},
	abstract = {<p>Identifying cis-regulatory elements is critical in understanding the direct and indirect interactions that occur within gene regulatory networks. Current approaches include DNase-seq, a technique that combines sensitivity to the nonspecific endonuclease DNase I with high-throughput sequencing to identify regions of regulatory DNA on a genome-wide scale. Yet, challenges still remain in processing recalcitrant tissues that have low DNA content. Here, we describe DNase I SIM (for Simplified In-nucleus Method), a protocol that simplifies and facilitates generation of DNase-seq libraries from plant tissues for high-resolution mapping of DNase I hypersensitive sites. By removing steps requiring the use of gel agarose plugs in DNase-seq, DNase I SIM reduces the time required to perform the protocol by at least 2 days, while also making possible the processing of difficult plant tissues including plant roots.</p>},
	isbn = {1940-6029 (Electronic)1064-3745 (Linking)},
	url = {https://link.springer.com/protocol/10.1007/978-1-4939-7125-1_10},
	author = {Filichkin, S. A. and Megraw, M.}
}
