|Title||The protein expression landscape of the Arabidopsis root.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Petricka, JJ, Schauer, MA, Megraw, M, Breakfield, NW, J Thompson, W, Georgiev, S, Soderblom, EJ, Ohler, U, Moseley, MArthur, Grossniklaus, U, Benfey, PN|
|Journal||Proc Natl Acad Sci U S A|
|Date Published||2012 May 1|
|Keywords||Arabidopsis, Arabidopsis Proteins, Base Sequence, Chromatography, Liquid, DNA Primers, Gene Expression Profiling, Plant Roots, Plants, Genetically Modified, Protein Array Analysis, Protein Interaction Mapping, Proteome, Proteomics, RNA, Plant, Tandem Mass Spectrometry|
Because proteins are the major functional components of cells, knowledge of their cellular localization is crucial to gaining an understanding of the biology of multicellular organisms. We have generated a protein expression map of the Arabidopsis root providing the identity and cell type-specific localization of nearly 2,000 proteins. Grouping proteins into functional categories revealed unique cellular functions and identified cell type-specific biomarkers. Cellular colocalization provided support for numerous protein-protein interactions. With a binary comparison, we found that RNA and protein expression profiles are weakly correlated. We then performed peak integration at cell type-specific resolution and found an improved correlation with transcriptome data using continuous values. We performed GeLC-MS/MS (in-gel tryptic digestion followed by liquid chromatography-tandem mass spectrometry) proteomic experiments on mutants with ectopic and no root hairs, providing complementary proteomic data. Finally, among our root hair-specific proteins we identified two unique regulators of root hair development.
|Alternate Journal||Proc. Natl. Acad. Sci. U.S.A.|
|PubMed Central ID||PMC3344980|
|Grant List||1P50-GM081883 / GM / NIGMS NIH HHS / United States |
F32GM086976 / GM / NIGMS NIH HHS / United States
The protein expression landscape of the Arabidopsis root.
Submitted by Megraw Lab Admin on Tue, 2016-07-12 22:10