%0 Journal Article %J Proc Natl Acad Sci U S A %D 2012 %T The protein expression landscape of the Arabidopsis root. %A Petricka, Jalean J %A Schauer, Monica A %A Megraw, Molly %A Breakfield, Natalie W %A Thompson, J Will %A Georgiev, Stoyan %A Soderblom, Erik J %A Ohler, Uwe %A Moseley, Martin Arthur %A Grossniklaus, Ueli %A Benfey, Philip N %K Arabidopsis %K Arabidopsis Proteins %K Base Sequence %K Chromatography, Liquid %K DNA Primers %K Gene Expression Profiling %K Plant Roots %K Plants, Genetically Modified %K Protein Array Analysis %K Protein Interaction Mapping %K Proteome %K Proteomics %K RNA, Plant %K Tandem Mass Spectrometry %X

Because proteins are the major functional components of cells, knowledge of their cellular localization is crucial to gaining an understanding of the biology of multicellular organisms. We have generated a protein expression map of the Arabidopsis root providing the identity and cell type-specific localization of nearly 2,000 proteins. Grouping proteins into functional categories revealed unique cellular functions and identified cell type-specific biomarkers. Cellular colocalization provided support for numerous protein-protein interactions. With a binary comparison, we found that RNA and protein expression profiles are weakly correlated. We then performed peak integration at cell type-specific resolution and found an improved correlation with transcriptome data using continuous values. We performed GeLC-MS/MS (in-gel tryptic digestion followed by liquid chromatography-tandem mass spectrometry) proteomic experiments on mutants with ectopic and no root hairs, providing complementary proteomic data. Finally, among our root hair-specific proteins we identified two unique regulators of root hair development.

%B Proc Natl Acad Sci U S A %V 109 %P 6811-8 %8 2012 May 1 %G eng %N 18 %R 10.1073/pnas.1202546109