TY - JOUR T1 - MicroRNA promoter element discovery in Arabidopsis. JF - RNA Y1 - 2006 A1 - Megraw, Molly A1 - Baev, Vesselin A1 - Rusinov, Ventsislav A1 - Jensen, Shane T A1 - Kalantidis, Kriton A1 - Hatzigeorgiou, Artemis G KW - Arabidopsis KW - Base Sequence KW - Binding Sites KW - Databases, Genetic KW - Feedback, Physiological KW - Genes, Plant KW - MicroRNAs KW - Promoter Regions, Genetic KW - TATA Box KW - Transcription Factors KW - Transcription Initiation Site AB -

In this study we present a method of identifying Arabidopsis miRNA promoter elements using known transcription factor binding motifs. We provide a comparative analysis of the representation of these elements in miRNA promoters, protein-coding gene promoters, and random genomic sequences. We report five transcription factor (TF) binding motifs that show evidence of overrepresentation in miRNA promoter regions relative to the promoter regions of protein-coding genes. This investigation is based on the analysis of 800-nucleotide regions upstream of 63 experimentally verified Transcription Start Sites (TSS) for miRNA primary transcripts in Arabidopsis. While the TATA-box binding motif was also previously reported by Xie and colleagues, the transcription factors AtMYC2, ARF, SORLREP3, and LFY are identified for the first time as overrepresented binding motifs in miRNA promoters.

VL - 12 IS - 9 ER -