TY - JOUR T1 - The protein expression landscape of the Arabidopsis root. JF - Proc Natl Acad Sci U S A Y1 - 2012 A1 - Petricka, Jalean J A1 - Schauer, Monica A A1 - Megraw, Molly A1 - Breakfield, Natalie W A1 - Thompson, J Will A1 - Georgiev, Stoyan A1 - Soderblom, Erik J A1 - Ohler, Uwe A1 - Moseley, Martin Arthur A1 - Grossniklaus, Ueli A1 - Benfey, Philip N KW - Arabidopsis KW - Arabidopsis Proteins KW - Base Sequence KW - Chromatography, Liquid KW - DNA Primers KW - Gene Expression Profiling KW - Plant Roots KW - Plants, Genetically Modified KW - Protein Array Analysis KW - Protein Interaction Mapping KW - Proteome KW - Proteomics KW - RNA, Plant KW - Tandem Mass Spectrometry AB -

Because proteins are the major functional components of cells, knowledge of their cellular localization is crucial to gaining an understanding of the biology of multicellular organisms. We have generated a protein expression map of the Arabidopsis root providing the identity and cell type-specific localization of nearly 2,000 proteins. Grouping proteins into functional categories revealed unique cellular functions and identified cell type-specific biomarkers. Cellular colocalization provided support for numerous protein-protein interactions. With a binary comparison, we found that RNA and protein expression profiles are weakly correlated. We then performed peak integration at cell type-specific resolution and found an improved correlation with transcriptome data using continuous values. We performed GeLC-MS/MS (in-gel tryptic digestion followed by liquid chromatography-tandem mass spectrometry) proteomic experiments on mutants with ectopic and no root hairs, providing complementary proteomic data. Finally, among our root hair-specific proteins we identified two unique regulators of root hair development.

VL - 109 IS - 18 ER -