02614nas a2200517 4500008004100000020004600041245014500087210006900232260001200301300001200313490000700325520091000332653004701242653005901289653002401348653003801372653002901410653001701439653002001456653002101476653002001497653003601517653003301553653003001586653004301616653004101659653004801700653003301748100001901781700001501800700001801815700001101833700001801844700001301862700002001875700001401895700001401909700002101923700001401944700002201958700001501980700001501995700001802010700001902028856004902047 2016 eng d a1878-1551 (Electronic)1534-5807 (Linking)00aEstablishment of Expression in the SHORTROOT-SCARECROW Transcriptional Cascade through Opposing Activities of Both Activators and Repressors0 aEstablishment of Expression in the SHORTROOTSCARECROW Transcript c12/2016 a585-5960 v393 a
Tissue-specific gene expression is often thought to arise from spatially restricted transcriptional cascades. However, it is unclear how expression is established at the top of these cascades in the absence of pre-existing specificity. We generated a transcriptional network to explore how transcription factor expression is established in the Arabidopsis thaliana root ground tissue. Regulators of the SHORTROOT-SCARECROW transcriptional cascade were validated in planta. At the top of this cascade, we identified both activators and repressors of SHORTROOT. The aggregate spatial expression of these regulators is not sufficient to predict transcriptional specificity. Instead, modeling, transcriptional reporters, and synthetic promoters support a mechanism whereby expression at the top of the SHORTROOT-SCARECROW cascade is established through opposing activities of activators and repressors.
10aArabidopsis Proteins/ genetics/ metabolism10aArabidopsis/ genetics/growth & development/ metabolism10aComputer Simulation10aGene Expression Regulation, Plant10aGene Regulatory Networks10aGenes, Plant10aGenes, Reporter10aGenes, Synthetic10aModels, Genetic10aPlant Roots/cytology/metabolism10aPlants, Genetically Modified10aPromoter Regions, Genetic10aRepressor Proteins/genetics/metabolism10aTrans-Activators/genetics/metabolism10aTranscription Factors/ genetics/ metabolism10aTwo-Hybrid System Techniques1 aSparks, E., E.1 aDrapek, C.1 aGaudinier, A.1 aLi, S.1 aAnsariola, M.1 aShen, N.1 aHennacy, J., H.1 aZhang, J.1 aTurco, G.1 aPetricka, J., J.1 aForet, J.1 aHartemink, A., J.1 aGordan, R.1 aMegraw, M.1 aBrady, S., M.1 aBenfey, P., N. uhttps://doi.org/10.1016/j.devcel.2016.09.031