02389nas a2200433 4500008004100000022001400041245007000055210006600125260001600191300000800207490000600215520115000221653001601371653002501387653003001412653002901442653001401471653001601485653003101501653002001532653002601552653003301578100002201611700001801633700001801651700002501669700001601694700001901710700001601729700001501745700002901760700001501789700002201804700001501826700001701841700002701858700002201885856004801907 2011 eng d a1744-429200aA stele-enriched gene regulatory network in the Arabidopsis root.0 asteleenriched gene regulatory network in the Arabidopsis root c2011 Jan 18 a4590 v73 a
Tightly controlled gene expression is a hallmark of multicellular development and is accomplished by transcription factors (TFs) and microRNAs (miRNAs). Although many studies have focused on identifying downstream targets of these molecules, less is known about the factors that regulate their differential expression. We used data from high spatial resolution gene expression experiments and yeast one-hybrid (Y1H) and two-hybrid (Y2H) assays to delineate a subset of interactions occurring within a gene regulatory network (GRN) that determines tissue-specific TF and miRNA expression in plants. We find that upstream TFs are expressed in more diverse cell types than their targets and that promoters that are bound by a relatively large number of TFs correspond to key developmental regulators. The regulatory consequence of many TFs for their target was experimentally determined using genetic analysis. Remarkably, molecular phenotypes were identified for 65% of the TFs, but morphological phenotypes were associated with only 16%. This indicates that the GRN is robust, and that gene expression changes may be canalized or buffered.
10aArabidopsis10aArabidopsis Proteins10aGene Expression Profiling10aGene Regulatory Networks10aMicroRNAs10aPlant Roots10aReproducibility of Results10aSystems Biology10aTranscription Factors10aTwo-Hybrid System Techniques1 aBrady, Siobhan, M1 aZhang, Lifang1 aMegraw, Molly1 aMartinez, Natalia, J1 aJiang, Eric1 aYi, Charles, S1 aLiu, Weilin1 aZeng, Anna1 aTaylor-Teeples, Mallorie1 aKim, Dahae1 aAhnert, Sebastian1 aOhler, Uwe1 aWare, Doreen1 aWalhout, Albertha, J M1 aBenfey, Philip, N uhttp://megraw.cgrb.oregonstate.edu/node/322